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. 2018 Jan 16;8:841. doi: 10.1038/s41598-018-19364-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Phase contrast image of HMLE cells expressing Snail-ER or Twist-ER at 0 and 8 days post 4-OHT treatment. Scale bar = 100 μm. (B) Flow cytometric profiles of CD44 and CD24 on Snail-ER or Twist-ER HMLE cells treated with 4-OHT for the indicated number of days. (C) Relative expression of the mRNAs encoding macroH2A1.1, macroH2A1.2, E-cadherin, vimentin, and Zeb1 in the cells described in (B) as determined by real-time RT-PCR. GAPDH was used as a housekeeping gene control to account for variability in template loading. (D) Western blot analysis of expression of E-cadherin, vimentin, macroH2A1.1 and macroH2A1.2, proteins in the HMLE-Snail-ER cells treated with 4-OHT for the indicated number of days. Histone H3 was used as a loading control. (E) Immunofluorescence images of HMLE-Twist-ER cells treated with 4-OHT for 16 days. Cells were stained using antibodies against macroH2A1.1, macroH2A1.2, E-cadherin, or vimentin. Scale bar = 100 μm.