Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 16;8:841. doi: 10.1038/s41598-018-19364-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(A) Western blot analysis of expression of macroH2A1.1, macroH2A1.2 proteins in the HMLE-Snail-ER or HMLE-Twist-ER cells transduced by the indicated retroviral vectors. Histone H3 was used as a loading control. (A,B) Phase contrast of HMLE-Twist-ER cells overexpressing macroH2A1.1, macroH2A1.2, mutant macroH2A1.1 (G224E and G314E) after 8-day 4-OHT treatment. Mesenchymal morphology observed in the vector control, macroH2A1.2 cells and mutant macroH2A1.1 cells, but not macroH2A1.1 cells. Scale bar = 100 μm. (B,C) Flow cytometric analysis of cell-surface markers, CD44 and CD24, in the indicated cells treated with 4-OHT for 8 days. (C,D) Quantification of HMLE-Snail-ER and HMLE-Twist-ER cells as described in (C). (n = 4 experiments for Snail-ER (p = 0.0129), 2 experiments for Twist-ER (p = 0.0478) – GFP vs. macroH2A1.1)).