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. 2018 Jan 16;8:818. doi: 10.1038/s41598-018-19315-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Foxo1 Pharmacological Inhibitor Reduces Th9 Cell Differentiation. (A) Naïve CD4⁺ T cells were pretreated with AS1842856 (10 μM) or control vehicle for 2 hours followed by cell polarization under Th9 cell condition for four days. The cytokine profile was analyzed by Luminex assay. (B) Th9 cells were differentiated for 4 days followed by the addition of the Foxo1 inhibitor AS1842856 for another round of four days. IL-9 secretion was analyzed by Luminex. Data are representative of three experiments with similar results. *p < 0.05 by Unpaired Student t test; NS: not significant.