Table 1.

Clock genes identified from Bactrocera oleae (and see Fig. 1).

Gene	Length	Putative protein coding and other details
per CDS	3105 bp	A protein of 1034 aa residues, containing two Nuclear Localization Signals (NLS, aa 72 to 78 and 691 to 718), two PER-ARNT-SIM domains (PAS-A, aa 193 to 242; PAS-B, aa 343 to 395) and a Cytoplasmic Localization Domain (CLD, aa 398 to 457). There is only a single Threonine-Glycine tandem repeat (T-G) in the B. oleae PER sequence (Supplementary Fig. S1)
cyc CDS	1206 bp	A 401 aa polypeptide containing a basic Helix-Loop-Helix motif (bHLH, aa 22 to 74) followed by PAS-A (aa 114 to 157) and PAS-B (aa 306 to 353) domains (Supplementary Fig. S2)
cry CDS	1644 bp	A 547 aa protein, in which the DNA photolyase activity domain (aa 4 to 195) and the FAD binding domain (aa 213 to 516) contains the conserved putative interaction sites for FAD (Flavin Adenine Dinucleotide; 13/14 identity), MTHF (methenyltetrahydrofolate; 7/7 identity) and CPD (cyclobutane pyrimidine dimers; 14/14 identity) (Supplementary Fig. S3)
Clk (5′Clk CDS)	990 bp	A 330 aa truncated peptide that contains most of the functional domains, such as bHLH (aa 13 to 58) and PAS (PAS-A, aa 92 to 140; PAS-B, aa 252 to 297), and has high sequence homology to Clk of D. melanogaster (Supplementary Fig. S4).