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. 2018 Jan 12;7:539. doi: 10.3389/fcimb.2017.00539

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Copyright © 2018 Rajwani, Yam, Zhang, Kang, Wong, Leung, Tam, Tulu, Zhu and Siu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reassessment of enhanced intracellular growth in THP-1 macrophage. Growth indexes showing multiplication of M. tuberculosis strains inside THP-1 macrophage. Higher growth index of hypervirulent strain H112 (red) relative to reference virulent strain H37Rv (black with squares), avirulent strain H37Ra (black with triangles), and control strains H54 (blue) were clearly demonstrated. Growth index was determined as mean CFU per ml at day 0, 3, 6 divided by mean CFU per ml at day 0, respectively. The lines represent mean of triplicate experiments.