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. 2018 Jan 16;8:823. doi: 10.1038/s41598-018-19341-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential miRNA expression in OSCC margins. (A) Venn diagram illustrating the relationship between differentially expressed miRNAs in the three discrete comparisons: T vs WL (Blue Circle), T vs NBI (Red Circle), WL vs NBI (Green Circle). For each group, upregulated miRNAs (red) and downregulated miRNAs (green) are tabulated. (B) Boxplots showing the expression profiles across the tumour margins of the 3 most upregulated and downregulated miRNA derived from the T vs NBI comparison. (C) Principal Component Analysis (PCA) plot of miRNA differential expression data showing spatial segregation according to biopsy location: T- tumour; WL – white light; NBI – narrow band imaging.