Table 2.
ZIKV-positive pools for the different mosquito species. CPE appearance times and Ct values by RT-qPCR.
| Mosquitoes genera and species | Number of mosquitoes | Pools presenting CPE | CPE (dpi) | Ct mean values in RT-qPCR for ZIKV (SD) |
|---|---|---|---|---|
| Aedes aegypti | 25 | Male | 2 | 10.50 (0.06) |
| Ae. aegypti | 8 | SG | 3 | 14.14 (1.40) |
| MG | 3 | 13.87 (0.67) | ||
| B | 3 | 13.39 (0.35) | ||
| Ae. aegypti | 3 | SG | 3 | 12.97 (1.07) |
| MG | 3 | 14.19 (0.95) | ||
| B | 3 | 14.26 (0.47) | ||
| Ae. vexans | 1 | SG | 3 | 11.56 (0.91) |
| MG | 3 | 12.17 (0.56) | ||
| B | 3 | 13.74 (0.09) | ||
| Culex quinquefasciatus | 25 | Male | 2 | 11.91 (1.18) |
| Cx. quinquefasciatus | 16 | SG | 3 | 14.36 (0.33) |
| MG | 3 | 14.32 (0.35) | ||
| B | 3 | 14.01 (0.18) | ||
| Cx. quinquefasciatus | 20 | SG | 3 | 12.76 (0.43) |
| MG | 5 | 12.16 (0.51) | ||
| B | 3 | 14.49 (0.14) | ||
| Cx. quinquefasciatus | 25 | SG | 3 | 13.23 (0.55) |
| MG | 3 | 12.76 (0.22) | ||
| B | 3 | 12.59 (0.24) | ||
| Cx. quinquefasciatus | 9 | SG | 1 | 12.91 (1.79) |
| MG | 1 | 12.74 (0.22) | ||
| B | 2 | 13.74 (0.79) | ||
| Cx. quinquefasciatus | 3 | SG | 1 | 10.54 (0.71) |
| MG | 3 | 11.80 (1.19) | ||
| B | 2 | 14.74 (0.18) | ||
| Cx. coronator | 2 | SG | 3 | 12.65 (2.69) |
| MG | 3 | 13.26 (0.40) | ||
| B | 3 | 13.56 (0.09) | ||
| Cx. tarsalis | 3 | SG | 4 | 12.16 (0.58) |
| MG | 4 | 11.86 (0.58) | ||
| B | 1 | 13.89 (0.18) |
All RT-qPCR reactions were performed in triplicate. Ct mean and standard deviation were calculated. SG, salivary gland; MG, midgut; B, body; CPE, cytopathic effect; dpi, days post-inoculation; SD, standard deviation.