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. 2018 Jan 16;8:834. doi: 10.1038/s41598-017-18785-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-treatment with glyburide and RA improves wound healing as compared to treatment of glyburide alone. (A) Left: Daily record of wound closure in HFD-mice treated with ctrl, glyburide, or glyburide/RA co-treatment. Data were presented as mean ± SD. A two-way ANOVA test was used *p < 0.05 (Ctrl vs. glyburide); ^†p < 0.05, ^††p < 0.01, ^†††p < 0.001 (Ctrl vs. glyburide + RA); ^#p < 0.05, ^##p < 0.01 (glyburide vs. glyburide + RA) (N = 6 in each group), Ctrl = Control. Right: Representative cutaneous wound on day 1, 3 and 7 after wound creation. (B) qPCR analyses of M1 and M2 markers in wound tissues was presented as mean ± SD. A two-way ANOVA test was used *p < 0.05, ***p < 0.001 (Ctrl vs. glyburide); ^†p < 0.05, ^†††p < 0.001 (Ctrl vs. glyburide + RA); ^#p < 0.05 (glyburide vs. glyburide + RA) (N = 6 in each group).