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. 2018 Jan 16;9:9. doi: 10.1038/s41467-017-01881-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — G-CSF levels are increased by the selective activation of mPFC to NAc projections. a Experimental design of projection-specific DREADD stimulation. Mice were injected with a retrograde traveling CAV2-Cre virus in the NAc and a Cre-dependent hM3Dq-DREADD virus in either the mPFC or the VTA to allow for the specific stimulation of either mPFC to NAc or VTA to NAc. b Csf3 (G-CSF) mRNA levels in the NAc were increased after mPFC to NAc stimulation (one-way ANOVA; F_(2,12) = 13.4, p = 0.0009, Sidak post-hoc: p = 0.0037 vs control). c Csf3r (G-CSFR) mRNA levels in the NAc were increased only after mPFC to NAc stimulation (F_(2,12) = 8.14, p = 0.0058, p = 0.0093 vs control). d Peripheral G-CSF serum levels were not affected by stimulation (F_(2,12) = 1.82, p = 0.20). e Mice were injected i.p. for 7 days with cocaine methiodide (CocMet), a cocaine analog that does not cross the blood brain barrier, to assess the effects of peripheral cocaine on G-CSF. Cocaine methiodide chronic treatment had no effect on f Csf3 (G-CSF) mRNA levels in the NAc (two-tailed Student’s t-test; t ₍₁₂₎ = 0.772, p = 0.45), g Csf3r (G-CSFR) mRNA levels in the NAc (t ₍₁₂₎ = 1.11, p = 0.29), or h G-CSF serum levels (t ₍₁₂₎ = 0.631, p = 0.54). Data represented as mean ± s.e.m. (**p < 0.01 for Sidak post-hoc tests)