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. 2018 Jan 16;8:862. doi: 10.1038/s41598-018-19262-9

Figure 1.

Figure 1

Inhibition of DEX induced Fibronectin (FN) expression by hGRβ overexpression in cultured MTM cells. Transduction of MTM cells with Ad5 vectors carrying null or hGRβ at different MOIs was detected by hGRβ western immunoblot and at mRNA levels. (A) hGRβ overexpression was detected at MOIs 50 and 100 in western immunoblotting. (B) qPCR showed increased expression of hGRβ; data are presented as means ± SEM, *P = 0.02, n = 3, unpaired student’s t-test. DEX treatment of MTM cells increased FN (C) expression, whereas transduction of MTM cells with Ad5.hGRβ inhibited DEX-induced FN expression as shown by immunocytochemistry. Control (CTRL), Ethanol (ETOH) and Ad5.Null treatments served as controls for these experiments. Green color represents FN staining (in Fig. 1B). Blue color represents DAPI staining showing cell nuclei. Actin served as loading control in Fig. 1A. 20× magnification. Representative data for 3 experimental triplicates.