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. 2018 Jan 16;8:845. doi: 10.1038/s41598-018-19223-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased cytosolic calcium level in response to supra-pharmacological capsaicin, irrespective of extracellular calcium. (A) HB2 brown preadipocytes loaded with Fluo-8 AM were treated with the indicated concentrations of capsaicin or A23187 (1 μM), and cytosolic calcium level was evaluated. (B) Effect of the depletion of extracellular calcium was evaluated. Cells were treated with vehicle, capsaicin (100 μM) or thapsigargin (100 nM).