Fig. 2.
Stereospecificity of the single additions to R(FR)3/(FE)4 amyloid. Reverse-phase HPLC chromatograms of the addition reactions of DL-phenylalanine with soluble substrate R(FR)3 (a) and amyloid R(FR)3/(FE)4 (b). The R(FR)3 concentration was 100 μM, the (FE)4 concentration was 130 μM and amino acids were at 25 μM (black), 50 μM (red), 100 μM (blue), 200 μM (green) of each enantiomer. The D-amino acid addition products are denoted with lower case letters. The single addiction product diastereomers are connected with a dashed line to illustrate differences in the yield of the products at different concentration of amino acid. The * indicates the C-terminal deamidated substrate that remained after purification. For a qualitative comparison of the specificity of addition of other amino acids, see Supplementary Fig. 3 and Supplementary Table 2