FIG 3.

HuR expression in C and TC pancreas. (A) Immunoblot of protein extracts pancreas from C and TC mice. GAPDH serves as gel loading control. (B) Pancreas sections from C and TC pancreas immunostained for HuR. Arrowheads indicate large amounts of HuR in islet cells. Asterisk indicates cytoplasmic HuR in acinus cells in TC pancreas. (C) Immunoblots of cytoplasmic and nuclear protein lysates prepared from C and TC pancreas. Loading control GAPDH was used for quantitative comparison of HuR amounts in C and TC cytoplasmic lysates (values indicated beneath HuR panel). GAPDH and lamin A/C were used as markers for cytoplasmic and nuclear extract preparations, respectively. Whole-mount GFP fluorescent images of the pancreas from which each preparation was made are shown below the cytoplasmic lamin A/C panel.