TABLE 4.
Antibodies used for Western blot analyses and immunostaining
| Antibody | Source | Product no. | Primary antibody dilution |
|---|---|---|---|
| Anti-HuR (3A2) (Western blots) | Santa Cruz | sc-5261 | 1:500–1:1,000 |
| Anti-HuR (19F12) (mouse pancreas immunostaining) | Clonegene | CGF12 | 1:5,000 |
| Anti-HuR (19F12) (human pancreas microarray IHC) | Molecular Probes | A21277 | 1:250 |
| Anti-GAPDH (6C5) | Ambion | AM4300 | 1:8,000 |
| Anti-lamin A/C | Santa Cruz | Sc-6215 | 1:500 |
| Anti-beta actin, clone AC-15 | Sigma-Aldrich | A5441 | 1:10,000 |
| Antiactin, alpha-smooth muscle | Sigma-Aldrich | A5228 | 1:1,000 |
| Anti-REG3G | Abcam | ab198216 | 1:250–1:500 |
| Antiadipsin (C3) | Santa Cruz | sc-373860 | 1:500 |
| Anti-Cox2 (EPR12012) | Abcam | ab179800 | 1:1,000 |
| Anti-PDCD4 (D29C6) XP | Cell Signaling | 9535 | 1:1,000 |
| Anti-collagen type I | Millipore | ABT123 | 1:2,000 |
| Anti-CD45 | BioLegend | 103121 | 1:200 |
| Anti-CD80 | Abcam | ab64116 | 1:1,000 |
| Anti-CD86 | Abcam | ab213044 | 1:1,000 |
| Anti-CD3 (SP7) | Abcam | ab16669 | 1:200 |
| Anti-TNF-α (52B83) | Abcam | ab1793 | 1:500 |
| Anti-HIF-1 alpha Ab-4 (Clone H1alpha67) | Thermo Scientific | MS-1164-P | 1:500 |
| Antivimentin (EPR3776) | Abcam | ab92547 | 1:2,000 |
| Anti-IL-6 (D5W4V) XP | Cell Signaling | 12912 | 1:500 |