FIG 5 .

Coimmunoprecipitation analysis revealed that the intact RNase III fold is required for robust association of KREPB9 and KREPB10 with editosomes in BF and PF. Western blots were probed with anti-V5 antibody to detect V5-tagged KREPB9 or KREPB10 wt (B9 WT or B10 WT), G270R or G238R mutants (B9 or B10 G-R, respectively), or G270V or G238V mutants (B9 or B10 G-V, respectively) in both input and anti-KREPA2 immunoprecipitations (anti-A2 IP) of editosomes from BF KREPB9 (A) or KREPB10 (B) null backgrounds. Note that the V5 signal was absent in parental cells (B9 null or B10 null). Anti-KREPA1, -KREPA2, -KREL1, and -KREPA3 antibodies were used to detect editosome complexes. Similar analyses were performed in the PF CN cell line background for KREPB9 (C) or KREPB10 (D).