FIG 6 .
Presence of PMNLs in C. albicans/S. aureus rechallenged, protected animals. Mice (n = 10/group) were given the primary challenge of C. dubliniensis/S. aureus and rechallenged with C. albicans/S. aureus 14 days later. Control mice (n = 10) received no primary challenge. (A) H&E-stained smears of PMNLs from peritoneal lavage fluid collected 4 h after rechallenge. The illustration is representative of several individual mice evaluated. (B) Flow cytometry analysis results of PMNLs from peritoneal lavage fluid prior to rechallenge through 96 h post-rechallenge with C. albicans/S. aureus. Percentages indicate proportions of Gr-1hi PMNLs present in the total cell population. MFI of Gr-1hi PMNLs within the encircled areas are shown in red. The illustration is representative of results for several individual mice evaluated. (C) Microbial burden (C. albicans and S. aureus) in peritoneal lavage fluid of mice 4 h post-rechallenge through 10 days post-rechallenge with C. albicans/S. aureus in those that remained alive. Data are cumulative for all animals from each group. Mφ, macrophage(s).