FIG 5 .

Analysis of pCS1-1 conjugative transfer of insertionally inactivated mutants of putative conjugation genes. Isogenic donor strains of each respective mutant are shown on the x axis. Wild-type transfer frequencies are illustrated for the tcsL TargeTron mutant “tcsLTT.” Shown are conjugation frequencies of cstD4 and cstB4 mutants and the corresponding complemented strains. The transfer frequency is expressed as the number of transconjugants per donor cell obtained from each mating. Means ± standard errors are shown; n ≥ 4. (V), strain containing vector plasmid pRPF185. Statistical analysis was carried out using a Mann-Whitney U test. *, P ≤ 0.05; **, P ≤ 0.01; ****, P ≤ 0.0001.