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. 2018 Jan 16;9(1):e01761-17. doi: 10.1128/mBio.01761-17

TABLE 1 .

ORFs within an ~17.3-kb region of pCS1-1 predicted to encode major components of a Gram-positive conjugation systema

Gene Predicted function Bioinformatic evidence
cstD4 Coupling protein C terminus containing pfam12696 domain; mediation of interaction of coupling proteins with cognate
relaxase (34); two predicted N-terminal transmembrane helices (3); Walker A and B motifs (61)
cstB6 Membrane pore
component
Nonspecific/superfamily domain (TIGR02783) found in TrbL (mating pair formation pore-forming protein) of trb
conjugation locus (34); 5–7 predicted transmembrane domains within the C-terminal half, 698 aa in length,
and therefore most similar to class DELTA VirB6-like proteins (3)
cstB4 VirB4-like ATPase Walker A and B motifs (62); multiple domains (pfam12846, TIGR02746, PRK13721) associated with
conjugation-related ATPases (34)
cstB1 Peptidoglycan
hydrolase
Short (22-aa) N-terminal sequence before single transmembrane domain; putative class ALPHA lytic
transglycolylase (3); N-terminal nonspecific domains (pfam01464, cd00254) associated with lytic
transglycolylases (34); C-terminal catalytic NlpC/P60 domain (34), also present in peptidoglycan hydrolase
TcpG from pCW3 conjugation system (63, 64)
cstD2 Relaxase Containing N-terminal catalytic tyrosine motifs and histidine triad motif of MobMG family of relaxases (clusters
within MobP relaxase family) (35, 65)
a

Gene names and predicted functions of their products are shown. The bioinformatic evidence for each ORF’s predicted function is provided together with appropriate references. aa, amino acid.