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. 2018 Jan 16;9(1):e01845-17. doi: 10.1128/mBio.01845-17

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FIG 2 — Accumulation of spontaneous Gac⁻ mutants in NYB cultures of P. protegens Pf-5 over time. Wild-type P. protegens Pf-5 was cultured in NYB for 6 dpi. (A and B) The percentage of spontaneous Gac⁻ mutants in the bacterial population (A) and the bacterial cell density (B) were recorded daily. In panel B, the cell density of Gac⁻ mutants at 1 dpi is shown as an open triangle, which represents the detection limit, as no Gac⁻ mutant was detected by screening more than 400 colonies. Each experiment had at least three replicates. Means ± standard errors (error bars) are shown.