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. 2018 Jan 16;9(1):e01845-17. doi: 10.1128/mBio.01845-17

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FIG 6 — Influence of interspecific competition on the accumulation of spontaneous Gac⁻ mutants of P. protegens Pf-5. Different inhibitory effects of the wild-type Pf-5 strain and the ΔgacA mutant of strain Pf-5 on the growth of B. subtilis strain 168 on NYA (A) and NYB (B). In panel B, strain 168 was inoculated in NYB or coinoculated with Pf-5 strains with an initial ratio 1:1 in NYB, and the cell density of strain 168 was tested at 6 dpi. (C) Cell density of P. protegens Pf-5 and the ΔgacA mutant grown in pure culture or in coculture with B. subtilis strain 168 in NYB at 6 dpi. P value was calculated by a Student’s t test. (D) The percentage of Gac⁻ mutants that accumulated in the pure culture of wild-type Pf-5 or the coculture of wild-type Pf-5 and strain 168 in NYB at 6 dpi is shown. Each experiment had at least three replicates. Means ± standard deviations are shown.