TABLE 1 .

Sites of mutations in spontaneous Gac⁻ mutants sampled from cultures of wild-type P. protegens Pf-5 and derivatives^a

Strain	Replicate	Colony	gacSb										gacAb
			M1A	T254S	T307P	A447V	A463D	L476F	Q727*	2419^2420 insG*	Δ500-504	Δ2422-2426	RBS	V4E	V4G	I29N	T103I	Q155R	K190R
Pf-5	1	1								X
	1	2																X
	2	1						X
	2	2				X
	3	1																X
	3	2						X
ΔofaA	1	1			X
	1	2			X
	2	1c
	2	2							X
	3	1										X
	3	2					X
ΔpltA	1	1	X
	1	2	X
	2	1												X
	2	2														X
	3	1													X
	3	2													X
LK147	1	1																	X
	1	2															X
	2	1									X
	2	2		X
	3	1											X
	3	2															X

^aP. protegens Pf-5 and derivatives (ΔofaA, ΔpltA, and the 6-fold mutant LK147) were each grown in three replicate flasks containing NYB for 4 days, dilutions of the cultures were spread on LMA, and two exoprotease-deficient colonies were selected from each replicate. gacS and gacA were PCR amplified and sequenced to map mutations.

^bAbbreviations of amino acids show the results of point mutations in the gacS or gacA gene. RBS indicates a mutation in the predicted ribosome binding site. An asterisk indicates a stop codon introduced by a point mutation or by an insertion.

^cNo mutation in gacS or gacA was found in this isolate.