TABLE 1 .

Summary of best-fit diffusion coefficients and fractional populations

EF-Tu or ribosome typea	Dmean (µm2/s)b	fslowc	Dslow (µm2/s)	Dfast (µm2/s)
Normal growth conditions
EF-Tu WTd	2.02 ± 0.19	0.60 ± 0.05	1.0 ± 0.2	4.9 ± 1.2
Ribosome WTe	0.4 ± 0.1	0.7 ± 0.05	0.1 ± 0.1	1.2 ± 0.5
EF-TuL148A mutantd	3.1 ± 0.3	0.3 ± 0.05	1.2 ± 0.5	4.5 ± 1.0
After Rif treatment
EF-Tu WTd	3.5 ± 0.4	0.35 ± 0.05	1.5 ± 0.5	4.9 ± 1.5
EF-TuL148A mutantd	5.2 ± 0.4	0.1 ± 0.05	1.9 ± 1.2	5.6 ± 1.2

^aNormal growth conditions were used, except for measurements after rifampin (Rif) treatment as noted.

^bMean diffusion coefficient estimated from first two points of MSD plot (Fig. 2).

^cBest-fit fractional population of the more slowly diffusing state. The fractional population of the more rapidly diffusing state is f_fast = 1 – f_slow.

^dC terminus labeled with mEos2.

^e30S subunits labeled by expression of the ribosomal protein S2-mEos2.