Skip to main content
. 2017 Dec 19;113(12):2634–2649. doi: 10.1016/j.bpj.2017.10.012

Figure 4.

Figure 4

Mobility profile of γ-secretase and the critical position and dynamics of HL1. (A) Distribution of residue MSFs for the intact γ-secretase in the membrane (NCT portion identical to the ANM curve is shown in red in Fig. 3B). Highest peaks in NCT are labeled, as well as sites serving as anchors or hinges (e.g., E245, F287, and E333), which exhibit small fluctuations in space. (B) Close-up view of key residues along PS1 MSF profile. PS1 loop HL1 (S104–T124) central portion exhibits large movements. The catalytic residues (D257 and D385) are highly stable (minima). (C) Location of HL1 loop residues (shown as red space-filling) and the broken TM6 (orange) of PS1 where D257 is located. All other structural elements are colored as in Fig. 1A. The location of NCT peak residues is indicated by the white circle. (D) Top view of the TMD (colored as in Fig. 1; NCT is removed for visual clarity). HL1 covers a large portion of the EC-facing vestibule of PS1. See Figs. 5 and S4 for more details.