Figure 5.

Transcriptional regulation of clf1 and protease activity in Δclf1. (A) Transcript abundance of clf1 in constant darkness (DD) or in constant light with 1,500 lux (“1,500”) or 5,000 lux (“5,000”). Strains were grown for 72 h on MA-media supplemented with 1% (w/v) cellulose in constant darkness (DD) or constant light at different light intensities. Two replicates for determination of cbh1 and env1 transcript abundance. (B) For determination of protease activity, strains were grown on milk TSA agar at 28°C in constant darkness (DD) or constant light (LL). Halo formation by proteases and hyphal extension was determined at the indicated time points. Halo diameter was related to the respective diameter of the mycelium under the respective condition. Ratio of halo to mycelia is given for the wild-type QM6a (dark gray) and Δclf1 (light gray). Three biological replicates and two technical replicates were used. Errorbars show standard deviations.^* indicates values significantly different to QM9414 in darkness (p < 0.05). Statistical significance of other comparison is given with p-values in the text.