Table 1.
Proteins identified by mass spectrometry from QM6a grown in constant light (LL, 1,500 lux) or constant darkness (DD).
| Protein ID | Band | Predicted size [kDa] | Isolated in | Gene name | Group | Family | Description | Domain annotation | Cellulase specific | Light regulated | Regulation by photoreceptors | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Δenv LL | Δenv DD | Δblr1 LL | Δblr1 DD | Δblr2 LL | Δblr2 DD | |||||||||||
| TR 123456 | band A | 117 | LL | CAZyme | GH 65 | candidate acid trehalase | pfam03632, pfam03636, pfam03633 | U | NR | NR | NR | NR | NR | NR | NR | |
| TR 64397 | band B | 78 | LL | Neutral/alkaline non-lysosomal ceramidase | pfam 04734 | U | NR | U | NR | U | NR | U | NR | |||
| TR 51365 | band B/J | 93 | DD LL | Protease | Peptidase S8 family protein, subtilase superfamily, PoS1 related | cd07489; pfam00082, pfam02225, pfam06280 | NR | NR | NR | NR | NR | NR | NR | NR | ||
| TR 121746 | band C | 83 | LL | gluc78 | CAZyme | GH 55 | candidate b-1,3-exoglucanase; potential pectate lyase; related to GLUC78 | pfam12708 | U | NR | D | NR | NR | NR | NR | NR |
| TR 82623 | band D | 65 | LL | Protease | Peptidase S53 family, sedolisin like | cd04056; pfam09286 | NR | D | NR | NR | NR | NR | U | U | ||
| TR 122780 | band D | 50 | LL | rgxl | CAZyme | GH 28 | candidate a-glycosidase related to exo-polygalacturonase | pfam00295 | U | NR | D | NR | NR | NR | NR | NR |
| TR 104461 | band E | 37 | LL | CAZyme | CE 1 | related to pimeloyl-ACP methyl ester carboxylesterase (COG0596), alpha/beta hydrolase superfamily (cl21494) | COG0596; cl21494; pfam00561 | NR | NR | NR | NR | NR | NR | D | NR | |
| TR 111442 | band F/R | 23 | DD LL | distantly related to the ceratoplatanin superfamily | cl06331 | D | NR | NR | NR | NR | NR | NR | NR | |||
| TR 72183 | band G | 24 | LL | Cyanovrin domain containing protein (pfam08881) | pfam08881 | NR | NR | U | NR | D | NR | D | NR | |||
| TR 119989 | band H | 9 | LL | hfb2 | hydrophobin | pfam06766 | NR | NR | D | NR | NR | NR | NR | NR | ||
| TR 122374 | band H | 13 | LL | unknown | unknown | U | NR | D | NR | NR | NR | NR | NR | |||
| TR 49081 | band I | 87 | DD | cel74a | CAZyme | GH 74 | candidate xyloglucanase; EGL6 (CEL74A) | pfam02012, pfam00734 | U | U | D | NR | D | NR | NR | NR |
| TR 121127 | band I/M | 87 | DD | bxll | CAZyme | GH 3 | b-xylosidase BXL1 | pfam01915, pfam00933 | U | U | D | NR | D | NR | NR | NR |
| TR 72526 | band J | 93 | DD | glrl | CAZyme | GH 67 | candidate a-glucuronidase GLR1 | pfam07488, pfam07477, pfam03648 | U | U | D | NR | D | NR | D | U |
| TR 76672 | band K | 78 | DD | bgl1/cel3a | CAZyme | GH 3 | b-glucosidase | pfam01915, pfam00933 | U | NR | D | NR | D | NR | NR | U |
| TR 123989 | band K | 54 | DD | cbh1/cel7a | CAZyme | GH 7 | cellobiohydrolase CBH1 | pfam00840, pfam00734 | U | NR | D | NR | D | NR | NR | NR |
| TR 111849 | band L | 53 | DD | xyn4 | CAZyme | GH 30 | xylanase 4 | pfam02055 | U | NR | D | NR | NR | NR | NR | NR |
| TR 123026 | band N | 36 | DD | transaldolase/ftuctose-6-phosphate aldolase (IPR001585) | pfam00923 | NR | NR | NR | NR | NR | NR | NR | NR | |||
| TR 4213 | band O | 28 | DD | ribonuclease T2 (cd01061) | cd01061, pfam00445 | U | NR | D | NR | NR | NR | NR | NR | |||
| TR 123244 | band O | 58 | DD | Protease | Peptidase S8 family, proteinase K like, subtilase like | pfam05922, pfam00082 | U | NR | NR | NR | NR | NR | NR | NR | ||
| TR 65406 | band P | 29 | DD | CAZyme | GH 16 | candidate b-glycosidase related to cell-wall modifying enzymes | pfam00722 | NR | NR | NR | NR | NR | NR | NR | NR | |
| TR 120961 | band Q | 27 | DD | cel61b | CAZyme | AA9 | lytic polysaccharide monooxygenase, endo-1,4-glucanase; CEL61B | pfam03443 | U | NR | D | NR | D | NR | NR | NR |
| TR 123818 | band S | 23 | DD | xyn2 | CAZyme | GH 11 | xylanase 2 | pfam00457 | U | U | D | NR | D | NR | D | NR |
| TR 122127 | band T | 20 | DD | putative cell wall protein related to A. nidulans PhiA | unknown | U | NR | D | NR | NR | NR | NR | NR | |||
| TR 111915 | band U | 15 | DD | Kazal domain containing protein (IPR011497) | IPR011497 | U | D | D | NR | U | NR | NR | NR | |||
| TR 108663 | band V | 14 | DD | unknown | unknown | U | NR | D | NR | NR | NR | NR | NR | |||
Protein IDs refer to the JGI genome annotation of T. reesei (http://genome.jgi.doe.gov/Trire2/Trire2.home.html). Similar band letters indicate that the band contained more than one protein. GH, glycosyl hydrolase; CE, carbohydrate esterase; NR, not regulated. Domain association as determined in the JGI database and with NCBI CDD search is provided. An overview on gene regulation in QM9414 in constant light (LL) or constant darkness (DD) upon growth on cellulose and in photoreceptor mutant strains lacking ENVOY (ENV1), Blue light regulator 1 (BLR1) or Blue Light Regulator 2 (BLR2) is provided. Therefore, data were taken from previous studies (Tisch and Schmoll, 2013; Tisch et al., 2014; Stappler et al., 2017) and are based on fold changes determined by ANOVA analysis of transcriptome abundance with a threshold of p-value 0.01. Detailed data including p-values are provided as Supplementary Material (Data sheet 2). Abbreviations: NR, no regulation; U, upregulation; D, downregulation.