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. 2018 Jan 12;8:1937. doi: 10.3389/fimmu.2017.01937

Figure 2.

Figure 2

CD19+CD21− B cells in patients with or without chronic graft-versus-host disease (cGvHD) and healthy controls (HC). (A) FACS plots showing CD21− B cell frequency in a representative “no GvHD” patient, GvHD patient, and a healthy control. Chronic GvHD patients (n = 32) have significantly higher percentage of circulating CD21− B cells compared with “no cGvHD” patients (n = 23) and HC (n = 18). (B,C) Color-coded gating on CD19+ B cells in representative “no GvHD” and active cGvHD patients; (B) cGvHD patient has higher frequencies of CD21− B cells (red) compared with the “no cGvHD” representative patient (yellow). (C) Gated CD19+ B cells from the same examples are shown; the majority of CD21− B cells in the “no cGvHD” group (yellow) fall within the CD24hiCD38hi transitional B cell and CD24−CD38hi plasmablast regions. Conversely, the majority of CD21− B cells in “cGvHD” patients (Red) fall within the CD24−CD38− exhausted B cell and CD24−CD38hi plasmablast regions. (D) Phenotypic characterization of transitional (yellow) and exhausted CD21− B cells (red) compared with CD21+ B cells (black) in a patient with cGVHD.