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Immunofluorescence staining was used to detect TSPAN1 protein expression in Eca-109 cells. TSPAN1 protein was stained with anti-TSPAN1 polyclonal antibody and marked by (A) tetramethylrhodaminein red, which was located in the cytoplasm. (B) The nuclei were stained with Hoechst-33258 in blue. (C) Merged figure. Magnification, ×400. TSPAN1, tetraspanin.
