Figure 3.
Expression of TSPAN1 mRNA in Eca-109 cells was detected by reverse transcription-quantitative polymerase chain reaction following siRNA transfection. The TSPAN1 mRNA level was significantly decreased in the TSPAN1 siRNA group compared with the negative control group (0.27±0.05 vs. 0.95±0.10; *P<0.05). TSPAN1, tetraspanin 1; siRNA, small interfering RNA; NC, negative control; Un, untreated; GD, GAPDH served as an internal control. GD, GAPDH.