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. 2017 Dec 13;8:1789. doi: 10.3389/fimmu.2017.01789

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Copyright © 2017 de Groot, Platteel, Kuijt, van Kooten, Vos, Sijts and van der Maaden.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative quantification image of the delivery of fluorescently labeled antigen into mouse ears. (A,B) An overlay of picture of the mouse ear and infrared fluorescence imaging. Two independent ear piercing experiments are shown for diphtheria toxoid (DT) (n = 2) (A) and tetanus toxoid (n = 3) (B). (C,D) Background fluorescence without and with mouse ear. (E) Gradient of solution containing 0.24, 0.6, and 1.2 Lf DT. Blue circles all indicate region of interest and have an equal diameter in all cases.