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Pipeline for the selection of 5′UTR candidates. Initial 5′UTR candidates were selected from transcriptomic data and then filtered for comparable expression level to adjacent mRNA, with length more than 35 bp. 5′UTR candidates that met these criteria were analyzed for homology with known RNA regulators by Rfam and for general conservation in other organisms by BLAST. From this analysis, 101 candidates were selected for experimental confirmation.
