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. 2017 Dec 12;7:511. doi: 10.3389/fcimb.2017.00511

Figure 2.

Figure 2

CPn0572 is associated with the host actin cytoskeleton in infected and transfected human cells. (A) Secreted CPn0572 colocalizes with host actin early in infection. HEp-2 cells expressing mCherry-actin (red) were infected with purified C. pneumoniae EBs (blue, MOI = 5). Secreted CPn0572 (green) is associated with actin recruitment to sites of EB entry at 30 min post-infection. The overall distributibbon of F-actin (red) is depicted in the merged image on the left; the boxed area is shown in greater detail on the right. (B) The effect of C. pneumoniae GFP-CPn0572 on the actin cytoskeleton differs from that of C. trachomatis GFP-Tarp. Upper row: Transfected HEK293T cells stained for GFP, GFP-CPn0572 or GFP-Tarp (green) and F-actin (red). The images shown are maximum intensity projections (MIP) of z-stacks. Lower row: A single section (Z: 7) from the z-stack of GFP-CPn0572 depicted in the upper row is shown. Note that not all GFP-CPn0572 filaments (arrows) coincide with actin fibers (arrowheads).