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. 2017 Dec 12;7:511. doi: 10.3389/fcimb.2017.00511

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Copyright © 2017 Zrieq, Braun and Hegemann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Displacement of mammalian cofilin from preassembled mammalian F-actin by GST–CPn0572 and GST-coupled domains of CPn0572 in vitro. (A) Binding of GST-CPn0572 and cofilin to pre-assembled F-actin was analyzed using a co-sedimentation assay. Supernatant (s) and pellet (p) fractions were analyzed by SDS-PAGE and stained with Coomassie blue. The presence (+) or absence (–) of each protein in the reaction mix is indicated. The corresponding protein band is marked. (B) The experiment was performed as described in (A), using 2 μM GST, GST-DUF (DUF) or GST-C-terminus (C-term.) or 4 μM GST-DUF (2x DUF). Supernatant (s) and pellet (p) fractions were analyzed by SDS-PAGE and stained with Coomassie blue. The presence or absence of each protein is indicated. The corresponding protein band is marked. A full-scale image of the original blot is presented in Figure S5.