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. 2017 Dec 20;11:411. doi: 10.3389/fncel.2017.00411

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Copyright © 2017 Wisse, Penning, Zaal, van Berkel, ter Braak, Polder, Kenney, Proud, Berkers, Altelaar, Speijer, van der Knaap and Abbink.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA and protein levels of candidate proteins in astrocyte cultures. (A) mRNA levels of the investigated candidate were investigated with qPCR. The mRNA levels were similar between wt and 2b5^ho astrocytes (n ≥ 5), with the exception of the Fndc3c1 mRNA, which was increased in 2b5^ho astrocytes. (B) The total protein levels of PROS1 and SLC3A2 were determined by Western blot. The graph shows the mean ± SD. The protein levels were increased in 2b5^ho cultures compared to wt cultures (n ≥ 3). ^∗∗p < 0.01. Post-translational modifications were overall not affected in 2b5^ho astrocytes. Western blot analyses did not show whether PROS1 in astrocytes was post-translationally modified or not. Staining of all proteins (loading control) is shown in Supplementary Figure 4.