Figure 4.

The effects of HFS diet, RW activity and age on mitochondrial proteins involved in substrate transport, OXPHOS pathway, fatty acid β‐oxidation, TCA cycle and antioxidant defence. (a) Summary of the linear regression analysis showing numbers of proteins uniquely affected by HFS diet, RW activity, and age with their overlap (Venn diagram), and direction (positive or negative) of the effect (table). (b) The correlation between the concentration of a specific mitochondrial protein and the mitochondrial O₂ flux driven by pyruvate plus malate (PM) in state 3. All time points are combined in the same plot. Only six proteins with the highest Pearson correlation coefficient are shown. Data are n = 4 mice per experimental group and time point. Experimental group abbreviations as in Figure 2