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. 2017 Dec 8;37(2):219–234. doi: 10.15252/embj.201797359

Figure 4. E‐Syts‐dependent DAG extraction from the PM in intact cells requires Ca2+ binding to C2 domains.

Figure 4

  1. Time‐course of normalized mCherry signal at the PM of WT and E‐Syts TKO cells expressing the DAG reporter C1PKC‐mCherry, as assessed by TIRF microscopy, in response to the indicated compounds.
  2. Quantification of F/F0 at the end of the experiment (arrow in A).
Data information: Mean and SEM, n = 25 cells (WT), n = 28 cells (TKO), n = 22 cells (TKO + EGFP‐E‐Syt1), n = 11 cells (TKO + EGFP‐E‐Syt1 C2Ax), n = 11 cells (TKO + EGFP‐E‐Syt1 C2Cx); P‐values from t‐test with Bonferroni corrections are quoted on the graphs. n.s., not significant; ***P < 0.001.