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. 2018 Jan 11;8:1954. doi: 10.3389/fimmu.2017.01954

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Copyright © 2018 Peres, Cunha, Barbosa, Santos, Oliveira, Jesus and de Almeida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) in Leishmania infantum. (A) Confocal microscopy showing the presence of E-NTPDase 1 and E-NTPDase 2 in L. infantum promastigotes. Parasites were fixed and labeled with primary rabbit antibody IgG anti E-NTPDase 1, anti E -NTPDase 2, or respective isotype IgG controls, followed by secondary labeling with anti-rabbit IgG linked to Alexa 488. (B) Western blot of the total extract from L. infantum labeled with antibody anti E-NTPDase 1, anti E-NTPDase 2, isotype control, and without antibody. Bars represent 10 µm.