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. 2018 Jan 11;8:1954. doi: 10.3389/fimmu.2017.01954

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Copyright © 2018 Peres, Cunha, Barbosa, Santos, Oliveira, Jesus and de Almeida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Leishmania infantum ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase) activity and macrophage infection during purine starvation and inhibition by suramin. (A) L. infantum E-NTPDase activity was evaluated by measuring the inorganic phosphate (Pi) in the extracellular medium of L. infantum promastigotes incubated with suramin, or under purine starvation, for 1 h. (B) Percentage of infected macrophages after 48 h. (C) Number of amastigotes per 100 macrophages after 48 h of incubation. Bars represent the mean ± SD of four independent experiments performed in triplicate.