Table 2.
Recovery of Bacillus anthracis Sterne 34F2 from non-sterile soil microcosms using tryptic soy agar (TSA) or polumixin B, lysozyme, EDTA and thallous acid (PLET) agar as the culture media (CFU g-1 soil).
Inoculum (spores g-1) | TSA |
PLET |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Total |
Spore |
Total |
Spore |
|||||||||
Mean | SD | % Rec | Mean | SD | % Rec | Mean | SD | % Rec | Mean | SD | % Rec | |
101 | 2.59 × 101 | 3.64 × 101 | 259.26 | 4.00 × 102 | 6.08 × 102 | 4000.00 | 0 | 0 | 0.00 | 6.00 × 102 | 8.74 × 102 | 6000.00 |
102 | 1.85 × 101 | 2.94 × 101 | 18.52 | 1.11 × 101 | 2.36 × 101 | 11.11 | 1.11 × 101 | 1.67 × 101 | 11.11 | 1.11 × 101 | 2.36 × 101 | 11.11 |
103 | 2.56 × 102 | 4.18 × 102 | 25.56 | 2.59 × 101 | 5.72 × 101 | 2.59 | 1.74 × 102 | 6.83 × 101 | 17.41 | 2.93 × 102 | 1.54 × 102 | 29.26 |
104 | 4.48 × 103 | 4.32 × 103 | 44.81 | 4.44 × 103 | 1.44 × 103 | 44.44 | 2.28 × 103 | 1.77 × 103 | 22.78 | 4.15 × 103 | 1.65 × 103 | 41.48 |
105 | 6.56 × 104 | 1.70 × 104 | 65.56 | 5.81 × 104 | 1.63 × 104 | 58.15 | 3.15 × 104 | 1.30 × 104 | 31.48 | 3.11 × 104 | 1.43 × 104 | 31.11 |
106 | 6.26 × 105 | 7.41 × 104 | 62.59 | 6.22 × 105 | 1.83 × 105 | 62.22 | 3.26 × 105 | 1.71 × 105 | 32.59 | 3.78 × 105 | 9.57 × 104 | 37.78 |
The results are presented as log10 values. Error is represented as standard deviation (arithmetic mean total viable count and spore count ± SD; n = 3). % Rec, recovery percentage.