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. 2018 Jan 8;11:426. doi: 10.3389/fncel.2017.00426

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Copyright © 2018 Prasuhn, Mårtensson, Krajka, Klein and Rakovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ectopic expression of TH is sufficient for dopamine synthesis in SH-SY5Y cells. (A) Western blot analysis of DDC, TH and GCH1 in human dermal fibroblasts, SH-SY5Y cells, and induced pluripotent stem cells (iPSCs)-derived neurons. β-actin served as a loading control. (B) Proteins from naive SH-SY5Y and SH-SY5Y cells expressing TH (SH^TH+) were extracted and analyzed using antibodies against TH, DDC and GCH1. Dopamine synthesis was detected only in SH^TH+ cells, but not in SH-SY5Y cells. BH4, tetrahydrobiopterin; TH, tyrosine hydroxylase; L- DOPA, L-3,4-dihydroxyphenylalanine; DDC, DOPA decarboxylase; GCH1, GTP cyclohydrolase 1; *non-specific band.