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. 2017 Dec 1;37(2):201–218. doi: 10.15252/embj.201797630

Figure 1. Generation of ICL in MEFs by Cre recombination.

Figure 1

  1. Schematic of the inverted lox P (iLoxP) site, flanked by sortable GFP and hCD2 markers.
  2. Model of reciprocal recombination yielding parental configurations 1/2′ and 1′/2 and dicentric/acentric configurations as a result of inverted recombination yielding configurations 1/1′ and 2/2′.
  3. Representative FACS plot showing loss of hCD2 marker expression of Ch14 ICL MEFs after treatment with 4‐hydroxy tamoxifen (4OHT) for 14 days to induce Cre, compared to ethanol‐treated control cells (See also Appendix Fig S1A).
  4. Shallow whole‐genome sequencing (copy number profiles) of large T antigen immortalized MEFs after exposure to Cre recombinase and sorted for control (hCD2 Plus) and ICL (hCD2 Minus) cells for chromosomes 12, 14 and 9 and 10, without any in vitro culturing, post‐FACS sort.