Table 4.
Sequences of cytokine and chemokine primers.
| Primer | Specificity | Sequence 5′–3′ |
|---|---|---|
| GAPDH | Bovine | F: GCG ATA CTC ACT CTT CTA CCT TCG A R: TCG TAC CAG GAA ATG AGC TTG AC |
| Human | F: TGG GTG TGA ACC ATG AGA AG R: GCT AAG CAG TTG GTG GTG C |
|
| IL-1β | Bovine | F: ACC TGA ACC CAT CAA CGA AAT G R: TAG GGT CAT CAG CCT CAA ATA ACA |
| Human | F: TGA TGG CTT ATT ACA GTG GCA ATG R: GTA GTG GTG GTG GGA GAT TCG |
|
| IL-8 | Bovine | F: CAC TGT GAA AAA TTC AGA AAT CAT TGT TA R: CTT CAC CAA ATA CCT GCA CAA CCT TC |
| Human | F: TCC TGA TTT CTG CAG CTC TGT R: AAT TTC TGT GTT GGC GCA GT |
|
| IL-10 | Bovine | F: GTG ATG CCA CAG GCT GAG AA R: TGC TCT TGT TTT CGC AGG GCA |
| Human | F: GGT TGC CAA GCC TTA TCG GA R: ACC TGC TCC ACT GCC TTG CT |
|
| IL-12 | Bovine/Human | F: GCA GCT TCT TCA TCA GGG ACA T R: CCT CCA CCT GCC GAG AAT T |
| INF-γ | Bovine | F: TTC TTG AAC GGC AGC TCT GAG R: TGG CGA CAG GTC ATT CAT CA |
| Human | F: CCA ACG CAA AGC AAT ACA TGA R: CCT TTT TCG CTT CCC TGT TTT |
|
| MCP1 | Bovine | F: GCT GTG ATT TTC AAG ACC ATC CT R: GGC GTC CTG GAC CCA TTT |
| Human | F: GTG CAG AGG CTC GCG AGC TA R: CAG GTG GTC CAT GGA ATC CTG |
|
| RANTES | Bovine | F: CAT GGC AGC AGT TGT CTT TAT CA R: CTC TCG CAC CCA CTT CTT CTC T |
| Human | F: AAC CCA GCA GTC GTC TTT GTC A R: CTC CCG AAC CCA TTT CTT CTC T |
|
| TGF-β | Bovine | F: GGC CCT GCC CTT ACA TCT G R: CGG GTT GTG CTG GTT GTA CA |
| Human | F: GGC CCT GCC CCT ACA TTT G R: CGG GTT ATG CTG GTT GTA CA |
|
| TNF-α | Bovine | F: TCT TCT CAA GCC TCA AGT AAC AAG T R: CCA TGA GGG CAT TGG CAT AC |
| Human | F: TCT TCT CGA ACC CCG AGT GA R: CCT CTG ATG GCA CCA CCA G |