Table 5.
Antibodies against MDM surface antigens used in this study.
| Antigen | Species | Antibody clone (source) | Isotype | Working dilution |
|---|---|---|---|---|
| CD14 | Bovine | CAM36A (VMRD, Pullman, WA, USA) | IgG1 | 1:500 |
| CD14 (FITC) | Human | 61D3 (Biozol) | IgG1 | 1:222 |
| CD40 | Bovine | IL-A156 (Dirk Werlinga) | IgG1 | 1:10 |
| CD40 (FITC) | Human | 5C3 (BD Biosciences) | IgG1κ | 1:222 |
| CD80 | Bovine | IL-A159 (Dirk Werling) | IgG1 | 1:10 |
| CD80 (FITC) | Human | MEM-233 (Biozol, Eching, Germany) | IgG1 | 1:222 |
| CD86 | Bovine | IL-A190 (Dirk Werlinga) | IgG1 | 1:10 |
| CD86 (FITC) | Human | BU63 (Biozol) | IgG1 | 1:222 |
| MHCI | Bovine | PT85A (VMRD, Pullman, WA, USA) | IgG2a | 1:500 |
| MHCI (FITC) | Human | G46-2.6 (BD Biosciences) | IgG1κ | 1:400 |
| MHCII | Bovine | H34A (VMRD, Pullman, WA, USA) | IgG2b | 1:500 |
| MHCII (R-PE) | Human | 423L (Biozol) | IgG2a | 1:400 |
a
Royal Veterinary College, Hatfield, UK.