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. 2017 Dec 19;113(12):2609–2620. doi: 10.1016/j.bpj.2017.10.026

Figure 2.

Figure 2

(A) Shown here are elution profiles of HMGA2 (coral), RF (teal), and complex (sea green) from the Superdex 75 10/300 GL, HiLoad 16/600 Superdex 75, and Superdex 75 10/300 GL SEC columns, respectively, indicating purity of both individual components as well as the complex. (B) Given here is EMSA indicating slower migration of RF (200 nM) upon interaction with HMGA2 (0–3 μM). (C) Shown here is the binding profile of HMGA2 with RF fit to a modified Hill equation. Data points are determined using densitometry and displayed as the average of three independent measurements (740 ± 50 nM). To see this figure in color, go online.