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. 2018 Jan 16;15:4. doi: 10.1186/s12986-018-0239-1

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Production of cytokines and chemokines by PBLs. Freshly isolated peripheral blood leukocytes were stimulated for 24 h with LPS/INF-γ in the presence of indicated amounts of LIG. Secreted chemokines, cytokines and differentiation factors (i.e. GM-CSF, IFN-gamma, CCL2/MCP-1, CXCL10/IP-105/RANTES (in a); TNF-alpha, IL-1beta, IL-6, CCL3/MIP1alpha, CCL5/RANTES (in b)) were measured by multiparametric analysis and are indicated in pg/mL. ‘LPS/INF-γ alone’ values for the molecules are indicated on the y-axis. Data are means ± SD of triplicate cultures of PBLs