Table 2.
Inflammatory proteins secreted by RAW264.7 cells
| Protein | Secretion by unstimulated cells [pg/mL] |
LPS-induced protein secretion
(ratio LPS-stim./unstim.) |
% inhibition by LIG (25 μM) |
% inhibition by
15-dPGJ 2 (1.25 μM) |
|---|---|---|---|---|
| CCL2/MCP-1 | 4005 | 65 | 86 ± 5 ***) | 58 ± 39 **) |
| CCL4/MIP-1β | 39,250 | 7.0 | 40 ± 13 *) | -9 ± 22 |
| CCL5/RANTES | 268 | 219 | 61 ± 4 **) | 2 ± 27 |
| IFN-γ | 22 | 4.2 | 32 ± 3 **) | 24 ± 4 *) |
| GM-CSF | 134 | 16 | 94 ± 5 **) | 91 ± 2 ***) |
| IL-1α | 9 | 233 | 95 ± 4 ***) | 50 ± 1 **) |
| IL-6 | 28 | 1442 | 90 ± 0 ***) | 76 ± 15 *) |
| IL-12p70 | 84 | 4.6 | 38 ± 2 **) | 27 ± 7 *) |
| TNF-α | 40 | 3820 | 68 ± 5 **) | 67 ± 2 **) |
RAW264.7 cells were stimulated with LPS and cultured for 24 h in the presence of substances. Secreted proteins were measured by multiparametric analysis (Luminex technology). The LPS-induced increase of secreted proteins is given as ratio of values obtained in stimulated versus unstimulated cells. * p < 0.05; ** p < 0.01; *** p < 0.005 (‘LPS + substance’ treated versus ‘LPS-only’ treated cells)