Fig. 3.

PtGAUT12.1 gene model, overexpression vector map, and GAUT12.1 transcript abundance in P. deltoides PtGAUT12-OE lines. A PtGAUT12.1 (Potri.001G416800) gene model from Phytozome 11.0 Populus trichocarpa v3.0. nt, nucleotides. B Structure of PtGAUT12.1 (Potri.001G416800) mRNA. CDS, coding sequence. C A schematic of the PtGAUT12.1 (Potri.001G416800) overexpression construct used to generate P. deltoides PtGAUT12.1 overexpression lines. D Relative GAUT12.1 and GAUT12.2 transcript abundance as determined by quantitative RT-PCR analysis of 3-month-old P. deltoides wild-type (WT) and PtGAUT12.1-OE lines. The 18S rRNA was used as the reference gene and the transcript expression of PdGAUT12.2 in AB29.12 was set to 1. Error bars represent SE, n = 6, *P < 0.05, **P < 0.001. Blue arrows indicate the three P. deltoides PtGAUT12.1-OE lines (AB29.2, AB29.7, and AB29.12) selected for further analyses