Table 1.
Glycosyl residue composition of alcohol insoluble residue (AIR) from stems of greenhouse-grown, 9-month-old P. deltoides WT, vector control, and PtGAUT12.1-OE lines of tetramethylsilane (TMS) derivatives
Glycosyl residue composition of AIR (mol% ± SE) | |||||||||
---|---|---|---|---|---|---|---|---|---|
Ara | Rha | Fuc | Xyl | GlcA | GalA | Man | Gal | Glc | |
WT | 3.06 ± 0.06 | 1.64 ± 0.06 | 0.09 ± 0.006 | 47.78 ± 1.21 | 0.21 ± 0.008 | 7.77 ± 0.21 | 6.08 ± 0.25 | 2.89 ± 0.14 | 30.48 ± 1.20 |
V Control-1 | 3.01 ± 0.08 | 1.79 ± 0.04 | 0.08 ± 0.004 | 47.26 ± 1.32 | 0.22 ± 0.006 | 6.97 ± 0.14 | 6.43 ± 0.28 | 3.01 ± 0.21 | 31.23 ± 1.32 |
AB29.2 | 2.96 ± 0.10 | 1.95 ± 0.06 | 0.07 ± 0.007 | 54.28 ± 1.01* | 0.20 ± 0.010 | 8.67 ± 0.22* | 4.11 ± 0.33* | 2.11 ± 0.16* | 25.65 ± 1.11* |
AB29.7 | 2.88 ± 0.07 | 2.01 ± 0.05 | 0.10 ± 0.006 | 55.64 ± 1.11* | 0.21 ± 0.005 | 8.96 ± 0.23* | 4.23 ± 0.27* | 1.97 ± 0.18* | 24.00 ± 1.21* |
AB29.12 | 2.57 ± 0.09 | 2.22 ± 0.08 | 0.08 ± 0.010 | 57.43 ± 0.98** | 0.19 ± 0.007 | 9.11 ± 0.24** | 3.15 ± 0.41** | 1.05 ± 0.21** | 24.20 ± 1.25* |
The amounts of sugar are represented as average mol% of AIR ± SE of 3 biological and 2 technical replicates (n = 6). Italics numbers with stars indicate mutant values that are significantly different from WT at * P ≤ 0.05, ** P ≤ 0.001 significant level (one-way ANOVA followed by Tukey’s multiple comparison test)