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. 2018 Jan 1;8(3):830–845. doi: 10.7150/thno.22012

Figure 6.

Figure 6

Effects of Tioconazole on Chemotherapy-Induced Apoptosis in Cancer Cells. (A) HCT116, (B) H4 and (C) MDA-MB-231 cells were treated with Dox (1 μM) for 24 h in the presence or absence of tioconazole (Tc, 40 μM), and apoptotic cells were stained with PI/AV. The apoptotic cells were analyzed and quantified with Prism 5.0. (D) The treated cells as in (A) were stained with JC-1 to determine the mitochondrial membrane potential. The representative data and quantitative results are shown in the left and right panels, respectively. (E) Untreated HCT116 cells or HCT116 cells treated with zVAD-FMK (50 μM) as described in (A) were harvested to assess caspase-3 activation with Caspase-Glo 3/7 luminescent assay. (F) HCT116 cells treated as in (C) were harvested for immunoblotting using antibodies against caspase-3 or PARP. (G) H4, HCT116 and MDA-MB-231 cells were pretreated with zVAD-FMK (50 μM), Tc (40 μM) or CQ (20 μM) prior to treatment with Dox (1 μM) for 24 h, and the cell viability was measured. The results are expressed as the mean ± SEM from at least 3 individual experiments. *p < 0.05; **p < 0.01; ***p < 0.001.