Fig. 2.

Enrichment in lymphocyte transcripts in immune infiltrates compared to the parenchyma. RNA was extracted and reverse-transcribed from laser-cut meningeal and WM perivascular immune infiltrates and from the adjacent GM and WM parenchyma, respectively. cDNA was preamplified for the indicated transcripts and analyzed by real-time PCR. Gene expression was evaluated in 29 (CD4, CD8, CD20) and 16 (CD138, CD68) matched immune infiltrates and parenchymal samples; significant differences between the two groups were assessed by Mann–Whitney test. Gene expression values are presented as 2^−ΔCt relative to GAPDH. The lines inside the boxes represent the median value; boxes extend from the 25th to the 75th percentile, covering the interquartile range (IQR), and whiskers extend from the 25th percentile − 1.5 IQR to the 75th percentile + 1.5 IQR. Maximum outliers outside the whiskers are represented by individual marks. n.s. not significant