Table-4.

PCR reaction mixture with sample from umbilicus.

Reagent in PCR kit	Amount
10 × RT buffer	5 µL
MgCl2	1.5 µL
dNTP	1 µL
GAPDH primer F/R	2 µL
Taq DNA polymerase enzyme	0.5 µL
cDNA from uterus/umbilicus	10µL
DEPCtreated water	30µL
Total	50 µL

PCR=Polymerase chain reaction